Linked Life Data

2466698

Source:http://linkedlifedata.com/resource/pubmed/id/2466698

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1989-4-28
pubmed:abstractText
Three monoclonal antibodies (mAbs) recognizing distinct epitopes on the delta-subunit of beef heart mitochondrial F1-ATPase were studied for their reactivity towards the delta-subunit both in isolated F1 and in the F0-F1 complex of submitochondrial particles. Two of the antibodies termed mAb delta 195 and mAb delta 239 had free access to delta in F1 and the F0-F1 complex. Partial hindrance was observed for the third antibody mAb delta 22. By a double antibinding assay, it was found that the binding sites for mAb delta 195 and mAb delta 239 were close to each other and possibly overlapping. Mapping studies conducted with the isolated delta-subunit showed that mAb delta 195 and mAb delta 239 interacted with the N-terminal portion of delta extending from Ala-1 to Met-16, whereas mAb delta 22 interacted with the fragment spanning Ser-17-Glu-68. It was concluded that the Ala-1-Met-16 segment of the delta-subunit in F1 and the F0-F1 complex is freely accessible from the outside, whereas the Ser-17-Glu-68 segment of delta is partially hidden, possibly as a result of interactions with other subunits.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
245
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
223-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Exploration of delta-subunit interactions in beef heart mitochondrial F1-ATPase by monoclonal antibodies.
pubmed:affiliation
Département de Recherche Fondamentale, Centre d'Etudes Nucléaires, Grenoble, France.
pubmed:publicationType
Journal Article, Comparative Study