Linked Life Data

2465412

Source:http://linkedlifedata.com/resource/pubmed/id/2465412

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-3-29
pubmed:abstractText
A voltage-dependent and Ca2+-activated cation channel found in the vacuolar membrane of the yeast, Saccharomyces cerevisiae, was incorporated into planar lipid bilayer and its gating characteristics were studied at the macroscopic and single-channel levels. The open-channel probability at steady state, which was estimated by the macroscopic current measurement, gave a maximum value at -10 mV and decreased in a graded fashion as the voltage became more positive or more negative. The steady-state voltage dependence was explained by assuming two independent gates, which had different rate constants and opposite voltage dependence. The fast-responding gate opened when the voltage of the cis side (the side to which the vesicles were added) was made more negative and the slow-responding gate behaved in the opposite direction. Relatively high concentrations of Ca2+, about 1 mM, were required on the cis side for opening the slow gate in a voltage-dependent manner. DIDS increased the open-channel probability of the fast gate when added to the cis side, but was ineffective on the slow gate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-2631
pubmed:author
pubmed:issnType
Print
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-55
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Gating behaviors of a voltage-dependent and Ca2+-activated cation channel of yeast vacuolar membrane incorporated into planar lipid bilayer.
pubmed:affiliation
Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't