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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1989-3-27
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pubmed:abstractText |
Human recombinant GM-CSF (rGM-CSF) was tested on highly purified and fractionated CFU-GM subsets. The fractionation was performed with the DS1-1 monoclonal antibody (MoAb), which distinguishes early and late CFU-GM. On whole bone marrow cells, rGM-CSF had a colony-stimulating activity comparable to that of known sources of CSFs, ie, the supernatant (SN) of TPA 30-1 or 5637 cell lines, used as control. A greatly reduced activity was observed when CFU-GM were depleted of phagocytizing and E rosetting cells (colony growth of 27% as compared with control). On fractionated CFU-GM, the rGM-CSF activity was even more reduced on both early and late progenitors (18% and 6% of colony growth, respectively). However, when rGM-CSF was used together with rG-CSF at suboptimal concentrations, the colony growth reached values analogous to that of control cultures. A synergistic interaction between rGM-CSF and rG-CSF in stimulating either early or late myeloid progenitors was observed. The results suggest that the activity of rGM-CSF on CFU-GM is mainly exerted through cooperation with accessory cells. r-G-CSF is one of the factors that can synergistically cooperate with r-GM-CSF in the myelopoietic stimulation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Colony-Stimulating Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating...,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
402-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2465035-Bone Marrow,
pubmed-meshheading:2465035-Cell Communication,
pubmed-meshheading:2465035-Cell Separation,
pubmed-meshheading:2465035-Colony-Forming Units Assay,
pubmed-meshheading:2465035-Colony-Stimulating Factors,
pubmed-meshheading:2465035-Drug Synergism,
pubmed-meshheading:2465035-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:2465035-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:2465035-Growth Substances,
pubmed-meshheading:2465035-Hematopoietic Stem Cells,
pubmed-meshheading:2465035-Humans,
pubmed-meshheading:2465035-Recombinant Proteins
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pubmed:year |
1989
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pubmed:articleTitle |
Granulocyte-macrophage colony-stimulating factor requires interaction with accessory cells or granulocyte-colony stimulating factor for full stimulation of human myeloid progenitors.
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pubmed:affiliation |
Dipartimento di Medicina, Università di Torino, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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