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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-3-16
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pubmed:abstractText |
We previously demonstrated that platelets express high levels of the tyrosine protein kinase pp60c-src. By a quantitative immunoblot assay, it is shown in this report that pp60c-src represents 0.2-0.4% of total platelet protein. The expression of high levels of pp60c-src in platelets correlated with high levels of total cell phosphotyrosine. Unstimulated platelets were shown to possess numerous phosphotyrosine-containing proteins by immunoblot analysis using antibodies that specifically recognize phosphotyrosine residues. To examine whether the pattern of phosphotyrosine-containing proteins changes upon platelet activation, lysates from thrombin- and phorbol ester-treated platelets were subjected to immunoblot analysis. Novel phosphotyrosine-containing proteins were detected within seconds following platelet stimulation. These results suggest that tyrosine phosphorylation, perhaps mediated by pp60c-src, may be involved in events associated with platelet activation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-169298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2419901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2424022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2426575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2427365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2427929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2434835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2446642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2463476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2464741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2582236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2981886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2992362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2994221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-2996780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-3039909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-3041347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-3262426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-3315856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-3464948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-3496339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-3502707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-3800947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-4039273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-4936093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6090944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6093480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6194438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6199661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6232463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6253989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6255214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6312092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6321473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-6601755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-7085651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464830-7199358
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
901-5
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2464830-Animals,
pubmed-meshheading:2464830-Blood Platelets,
pubmed-meshheading:2464830-Cell Line,
pubmed-meshheading:2464830-Humans,
pubmed-meshheading:2464830-Immunoblotting,
pubmed-meshheading:2464830-Phosphorylation,
pubmed-meshheading:2464830-Phosphotyrosine,
pubmed-meshheading:2464830-Protein-Tyrosine Kinases,
pubmed-meshheading:2464830-Proto-Oncogene Proteins,
pubmed-meshheading:2464830-Proto-Oncogene Proteins pp60(c-src),
pubmed-meshheading:2464830-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2464830-Thrombin,
pubmed-meshheading:2464830-Tyrosine
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pubmed:year |
1989
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pubmed:articleTitle |
Thrombin treatment induces rapid changes in tyrosine phosphorylation in platelets.
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pubmed:affiliation |
Department of Microbiology, State University of New York, Stony Brook 11794.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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