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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-3-21
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pubmed:databankReference |
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pubmed:abstractText |
The expression of the v-rel oncogene of avian reticuloendotheliosis virus (REV-T) transforms and immortalizes very immature avian lymphoid cells. In REV-T-transformed lymphoid cells which were persistently infected with reticuloendotheliosis-associated virus (REV-A), the REV-T proviral copy number increases after the initial integration event. In 23 independently derived REV-T-transformed cell lines, 15 of the 18 virus-producing cell lines had acquired additional proviruses. The rate at which the newly acquired proviral sequences accumulated differed for various cell lines. In some cell lines, additional REV-T proviral copies could be detected as early as 8 months after the initial integration event. A correlation exists between the number of REV-T proviral sequences and the length of time which a given cell line had been propagated in culture. The integration sites occupied by the newly acquired REV-T proviruses were distinct. In contrast, reticuloendotheliosis-associated virus proviral sequences in these REV-T-transformed virus-producing lymphoid cells did not increase during in vitro culture. Furthermore, the acquisition of additional REV-T proviral sequences did not occur in non-virus-producing cell lines. Two of the newly acquired proviral sequences were molecularly cloned and analyzed by restriction endonuclease mapping. Although the newly acquired REV-T proviruses have not sustained major deletions, the viral sequences and the v-rel oncogene display numerous restriction enzyme polymorphisms. The cellular flanking sequences of two newly acquired REV-T proviruses analyzed were unique and shared no homology with flanking sequences of the other REV-T proviruses in these transformed cells. The nucleotide sequence of the virus-cellular DNA junctions of one newly acquired provirus and its cellular sequence prior to proviral integration were defined. A 5-base-pair direct repeat of cellular origin was present on each side of the long terminal repeat, indicating that the mechanism of acquisition of additional REV-T proviral sequences used reverse transcription and integration of new REV-T proviral copies.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-180968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-219596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-225560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-2829193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-322278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-4443191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-4855738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-51935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6243436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6250038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6254044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6269747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6274086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6275117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6282448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6283142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6296425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6297744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6317898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6318091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2464702-6378386
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1107-15
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2464702-Animals,
pubmed-meshheading:2464702-Base Sequence,
pubmed-meshheading:2464702-Cell Transformation, Viral,
pubmed-meshheading:2464702-Chickens,
pubmed-meshheading:2464702-Cloning, Molecular,
pubmed-meshheading:2464702-DNA, Viral,
pubmed-meshheading:2464702-Gene Amplification,
pubmed-meshheading:2464702-Lymphocytes,
pubmed-meshheading:2464702-RNA-Directed DNA Polymerase,
pubmed-meshheading:2464702-Recombination, Genetic,
pubmed-meshheading:2464702-Restriction Mapping,
pubmed-meshheading:2464702-Reticuloendotheliosis virus,
pubmed-meshheading:2464702-Retroviridae,
pubmed-meshheading:2464702-Time Factors
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pubmed:year |
1989
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pubmed:articleTitle |
Acquisition of new proviral copies in avian lymphoid cells transformed by reticuloendotheliosis virus.
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pubmed:affiliation |
Department of Microbiology, University of Texas, Austin 78712-1095.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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