Linked Life Data

2464636

Source:http://linkedlifedata.com/resource/pubmed/id/2464636

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1989-3-13
pubmed:abstractText
The response of T cells to minor lymphocyte-stimulating locus (Mls) determinants remains poorly understood with respect to the antigenic determinants responsible for T cell stimulation and the types of APC capable of stimulating the response. In this report, we demonstrate that highly purified dendritic cells (DC) as well as B cells have the capacity to stimulate Mls-specific responses. Unseparated spleen cells, purified DC, resting B cells, and activated B cells were compared for their capacity to stimulate several Mls-reactive T cell hybridomas. Whereas the entire panel of Mls-reactive T cell hybridomas was stimulated strongly by unseparated spleen cells and activated B cells, the hybridomas responded only weakly to purified DC or resting B cells. Activation of resting B cells with either B cell stimulatory factor-1 (1 day pre-treatment) or LPS/dextran (2 or 3 day pre-treatment) greatly augmented their Mls-stimulatory capacity. In contrast, the Mls-stimulatory capacity of DC was not augmented by a 1-day pre-treatment with either B cell stimulatory factor-1 or supernatant from the DC-induced primary anti-Mls-MLR. In the primary anti-Mls-MLR, both purified DC and LPS/dextran-stimulated B blasts were found to elicit vigorous T cell proliferative responses. Much weaker responses were elicited by unseparated spleen cells. The stimulation of the primary anti-Mls-MLR by purified DC was further confirmed by producing Mls-specific T cell clones which were preferentially stimulated by DC. Autologous (Mlsb) DC were found to markedly enhance the primary anti-Mls-MLR response to small numbers of Mlsa B blasts. Thus, DC possess other "accessory cell" properties that augment the primary anti-Mls-MLR despite the predicted low level of Mls determinant expression on DC based on the results obtained with Mls-reactive hybridomas. Possible accessory cell properties of DC relevant to this phenomenon are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1069-78
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2464636-Animals, pubmed-meshheading:2464636-Antigens, Surface, pubmed-meshheading:2464636-B-Lymphocytes, pubmed-meshheading:2464636-Cell Separation, pubmed-meshheading:2464636-Dendritic Cells, pubmed-meshheading:2464636-Dextrans, pubmed-meshheading:2464636-Hybridomas, pubmed-meshheading:2464636-Interphase, pubmed-meshheading:2464636-Lipopolysaccharides, pubmed-meshheading:2464636-Lymphocyte Activation, pubmed-meshheading:2464636-Lymphocyte Culture Test, Mixed, pubmed-meshheading:2464636-Mice, pubmed-meshheading:2464636-Mice, Inbred AKR, pubmed-meshheading:2464636-Mice, Inbred BALB C, pubmed-meshheading:2464636-Mice, Inbred C3H, pubmed-meshheading:2464636-Mice, Inbred CBA, pubmed-meshheading:2464636-Mice, Inbred DBA, pubmed-meshheading:2464636-Minor Lymphocyte Stimulatory Antigens, pubmed-meshheading:2464636-Phenotype, pubmed-meshheading:2464636-T-Lymphocytes
pubmed:year
1989
pubmed:articleTitle
The role of dendritic cells as stimulators of minor lymphocyte-stimulating locus-specific T cell responses in the mouse. I. Differential capacity of dendritic cells to stimulate minor lymphocyte-stimulating locus-reactive T cell hybridomas and the primary anti-minor lymphocyte-stimulating locus mixed lymphocyte reaction.
pubmed:affiliation
Anna Perahia Addato Clinical Research Facilities, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.