Linked Life Data

2464163

Source:http://linkedlifedata.com/resource/pubmed/id/2464163

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-3-1
pubmed:abstractText
This paper describes an economical attachment substratum for use in patch-clamp experiments on cultured epithelial cells. It consists of Cytodex microcarrier beads on which growing cells present a unique profile perspective that increases the ease with which patch-clamp seals can be obtained. Cells from the mouse mandibular gland cell line, ST885, were grown on the beads and studied using standard patch-clamp techniques. The results were indistinguishable from those obtained from cells grown in monolayers on Petri dishes. The method can be applied in many types of patch-clamp experiment and used with a wide variety of cell types. It is especially useful if one wishes to do experiments in the cell-attached or whole-cell configurations and needs to add drugs or hormones to the external surface of the cell.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
413
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
90-2
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
The use of cytodex microcarrier beads in patch-clamp studies on cultured epithelial cells.
pubmed:affiliation
Department of Physiology, University of Sydney, NSW, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't