Linked Life Data

2464152

Source:http://linkedlifedata.com/resource/pubmed/id/2464152

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-3-1
pubmed:abstractText
A clone of the human transforming Ha-ras oncogene (pT24-C3) was methylated "in vitro" at its HpaII and HhaI sites and co-transfected with a selectable marker into NIH/3T3 cells. In the resulting colonies the normal or transformed morphology correlated respectively with a methylated or unmethylated status of the Ha-ras 1 promoter. A transfected, morphologically normal NIH/3T3 colony treated with 5'-azacytidine acquired a transformed phenotype growing in agar and in nude mice and showed demethylation of the promoter region of Ha-ras 1 gene. The cells of the reactivated colony produced human Ha-ras 1 mRNA and p21, and their DNA displayed transforming activity for NIH/3T3 recipient cells that was due to the original human Ha-ras 1 oncogene. We conclude that the methylation status of the promoter region of the human Ha-ras 1 modulates the expression and consequently the transforming activity of the oncogene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0890-6467
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-203
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Modulation of the human Harvey-ras oncogene expression by DNA methylation.
pubmed:affiliation
Division of Experimental Oncology, Istituto Nazionale Tumori, Milan, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't