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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1989-2-10
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pubmed:abstractText |
Incubation of calf pancreas microsomes with synthetic alpha-D-Manp-(1----6)-beta-D-Manp-(1----4)-beta-D-GlcpNAc-(1 ----4)-alpha-D- GlcpNAc-PP-Dol and GDP-D-[14C]-mannose gave three major lipid-linked oligosaccharide diphosphates. After release of the phospholipid residue by mild acid hydrolysis, the corresponding [14C]oligosaccharides were analyzed by gel-filtration, liquid chromatography, degradation by endo-N-acetyl-beta-D-glucosaminidases D and H, by jack bean alpha-D-mannosidase and Aspergillus oryzae (1----2)-alpha-D-mannosidase, acetolysis, and binding to concanavalin A-Sepharose. From the results it could be inferred that the following reaction took place in calf pancreas microsomes: alpha-D-Manp-(1----6)-beta-D-Manp-(1----4)-beta-D-GlcpNAc-(1 ----4)-alpha-D- GlcpNAc-PP-Dol + GDP-D-Man gave GDP + alpha-D-Manp-(1----3)-[alpha-D-Manp-(1----6)]-beta-D-Manp -(1----4)- beta-D-GlcpNAc-(1----4)-alpha-D-GlcpNAc-PP-Dol. The next products to be formed were alpha-D-Manp-(1----2)-alpha-D-Manp-(1----3)-[alpha-D-Manp -(1----6)]-beta-D- Manp-(1----4)-beta-D-GlcpNAc-(1----4)-alpha-D-GlcpNAc-PP-Dol, followed by alpha-D-Manp-(1----2)-alpha-D-Manp-(1----2)-alpha-D-Manp+ ++-(1----3)- [alpha-D-Manp-(1----6)]-beta-D-Manp-(1----4)-beta-D-GlcpNAc- (1----4)-alpha- D-GlcpNAc-PP-Dol. The mannose incorporation was enhanced by Triton X-100 and inhibited by Mn2+, and it occurred in the presence of either Mg2+ or EDTA. It is likely that the mannose donor was GDP-mannose since, under the conditions used, the formation of dolichyl mannosyl phosphate was negligible and the dolichyl heptasaccharide diphosphate accumulated.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylglucosaminidase,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Diphosphate Mannose,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Mannosidases,
http://linkedlifedata.com/resource/pubmed/chemical/Mannosyl-Glycoprotein...,
http://linkedlifedata.com/resource/pubmed/chemical/Mannosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Polyisoprenyl Phosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Polyisoprenyl Phosphate Sugars,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Mannosidase,
http://linkedlifedata.com/resource/pubmed/chemical/dolichyl diphosphate...
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0008-6215
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
179
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
381-92
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2463085-Acetylglucosaminidase,
pubmed-meshheading:2463085-Animals,
pubmed-meshheading:2463085-Carbohydrate Conformation,
pubmed-meshheading:2463085-Carbohydrate Sequence,
pubmed-meshheading:2463085-Cattle,
pubmed-meshheading:2463085-Chromatography, Gel,
pubmed-meshheading:2463085-Chromatography, Thin Layer,
pubmed-meshheading:2463085-Guanosine Diphosphate Mannose,
pubmed-meshheading:2463085-Hydrogen-Ion Concentration,
pubmed-meshheading:2463085-Kinetics,
pubmed-meshheading:2463085-Lipid Metabolism,
pubmed-meshheading:2463085-Magnesium,
pubmed-meshheading:2463085-Mannosidases,
pubmed-meshheading:2463085-Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase,
pubmed-meshheading:2463085-Mannosyltransferases,
pubmed-meshheading:2463085-Microsomes,
pubmed-meshheading:2463085-Molecular Sequence Data,
pubmed-meshheading:2463085-Oligosaccharides,
pubmed-meshheading:2463085-Pancreas,
pubmed-meshheading:2463085-Polyisoprenyl Phosphate Oligosaccharides,
pubmed-meshheading:2463085-Polyisoprenyl Phosphate Sugars,
pubmed-meshheading:2463085-alpha-Mannosidase
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pubmed:year |
1988
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pubmed:articleTitle |
Biosynthesis, in calf pancreas microsomes, of three lipid-linked oligosaccharide diphosphates from a synthetic dolichyl diphosphate tetrasaccharide.
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pubmed:affiliation |
Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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