Linked Life Data

2462705

Source:http://linkedlifedata.com/resource/pubmed/id/2462705

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-2-6
pubmed:abstractText
Quiescent Swiss 3T3 cells can be stimulated to reinitiate DNA synthesis through specific intracellular signalling pathways which include an increase in cyclic AMP levels and the activation of protein kinase C. In the present study we have compared the effect of these two pathways on the expression of c-fos and c-myc in Swiss 3T3 cells. In cells treated with the cyclic AMP-elevating agents forskolin or prostaglandin E1 or with the permeable cyclic AMP analogue, 8-bromo cyclic AMP, the levels of c-fos messenger RNA were 5 to 10% of those induced by bombesin, which activates protein kinase C. In contrast, a dramatic and comparable increase in the expression of c-myc messenger RNA was observed when cells were treated either with the cyclic AMP-increasing agents or with bombesin. Thus an increase in cyclic AMP leads to the differential induction of c-fos and c-myc messenger RNA in Swiss 3T3 cells. Significantly, we obtained comparable results when cells were treated with a mitogenic combination of cyclic AMP and insulin. Furthermore, these findings have been confirmed at the protein level. We concluded that the large induction of c-fos can be dissociated from cyclic AMP-mediated mitogenesis in Swiss 3T3 cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0890-6467
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-6
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Differential induction of c-fos and c-myc by cyclic AMP in Swiss 3T3 cells: significance for the mitogenic response.
pubmed:affiliation
Imperial Cancer Research Fund, London, United Kingdom.
pubmed:publicationType
Journal Article