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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1989-2-7
|
| pubmed:abstractText |
The human U937 cell line, a promonocyte-like leukemic line, has been shown to synthesize neutrophil elastase (Senior, R. M. et al. (1982) J. Clin. Invest. 69, 384-393). We have constructed a lambda gt11 cDNA library using RNA from this cell line and isolated a clone encoding part of the protein sequence of neutrophil elastase. Nucleotide sequencing indicates the clone encodes residues 108-243 (standard chymotrypsinogen numbering) of the enzyme, plus a hitherto unsuspected 20 residue-C-terminal extension of unusual structure. Comparison of the nucleotide and amino-acid sequence of elastase with the recently reported sequence of medullasin, a proteinase isolated from bone marrow, suggests they are identical.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0177-3593
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
369 Suppl
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3-7
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2462434-Amino Acid Sequence,
pubmed-meshheading:2462434-Base Sequence,
pubmed-meshheading:2462434-Cell Line,
pubmed-meshheading:2462434-Cloning, Molecular,
pubmed-meshheading:2462434-DNA, Recombinant,
pubmed-meshheading:2462434-Humans,
pubmed-meshheading:2462434-Molecular Sequence Data,
pubmed-meshheading:2462434-Neutrophils,
pubmed-meshheading:2462434-Oligonucleotides,
pubmed-meshheading:2462434-Pancreatic Elastase,
pubmed-meshheading:2462434-RNA
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Molecular cloning of human neutrophil elastase.
|
| pubmed:affiliation |
Department of Biochemistry, University of Georgia, Athens.
|
| pubmed:publicationType |
Journal Article
|