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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1989-1-23
|
| pubmed:databankReference | |
| pubmed:abstractText |
It has previously been shown that a mouse monoclonal antibody, designated 4E, reacts with an epitope common to all HLA-B and -C antigens and those of the HLA-Aw19 cross-reactive group, namely, HLA-A29, -A30, -A31, -A32, -Aw33, and -Aw74. In order to pinpoint the amino acid residues which comprise the public specificity recognized by 4E, and HLA-A29 cDNA clone was isolated and its predicted amino acid sequence compared with those of other cloned HLA class I genes. The isolated HLA-A29 cDNA corresponded to the rarer of the two A29 variant alleles, A29.1. Two amino acid residues of HLA-A29.1, gln-144 and arg-151, were found in all 24 HLA-B and HLA-C alleles examined but were present in only one of 15 HLA-A alleles for which sequence data are available. Importantly, this exceptional allele was HLA-A32, another member of the HLA-Aw19 cross-reactive group. Gln-144 and arg-151 should be capable of jointly contributing to the binding site for 4E, as they are situated in successive alpha-helical subregions and are predicted to be juxtaposed in the three-dimensional HLA molecule. Four other residues in the first or second external domains of HLA-A29.1 (thr-9, leu-62, gln-63, and his-102) were unique among the HLA-A alleles, but none of these was found in corresponding positions of HLA-B of -C alleles and thus failed to correlate with presence or absence of the 4E determinant. These observations are consistent with the notion that gln-144 and arg-151 define a determinant common to HLA-B, HLA-C, and the HLA-Aw19 cross-reactive group and the binding site of the monoclonal antibody 4E.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-B Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-C Antigens
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0093-7711
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
25-32
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2461903-Amino Acid Sequence,
pubmed-meshheading:2461903-Animals,
pubmed-meshheading:2461903-Antibodies, Monoclonal,
pubmed-meshheading:2461903-Base Sequence,
pubmed-meshheading:2461903-Cloning, Molecular,
pubmed-meshheading:2461903-DNA Probes,
pubmed-meshheading:2461903-Epitopes,
pubmed-meshheading:2461903-HLA Antigens,
pubmed-meshheading:2461903-HLA-B Antigens,
pubmed-meshheading:2461903-HLA-C Antigens,
pubmed-meshheading:2461903-Humans,
pubmed-meshheading:2461903-Immunoblotting,
pubmed-meshheading:2461903-Isoelectric Focusing,
pubmed-meshheading:2461903-Mice,
pubmed-meshheading:2461903-Molecular Sequence Data,
pubmed-meshheading:2461903-Peptide Mapping,
pubmed-meshheading:2461903-Precipitin Tests,
pubmed-meshheading:2461903-Transfection
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Molecular mapping of a new public HLA class I epitope shared by all HLA-B and HLA-C antigens and defined by a monoclonal antibody.
|
| pubmed:affiliation |
Laboratory of Human Immunogenetics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|