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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1988-12-20
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pubmed:abstractText |
A segment of the largest open reading frame of hepatitis B virus (HBV) was inserted into an open reading frame vector directing the expression in Escherichia coli of a fusion molecule containing 143 HBV-encoded amino acids. The fusion protein was used to generate antiserum which served in immunoblots to identify a polypeptide with a molecular mass of 65 kilodaltons in HBV particles. Because of the small number of molecules in virus particles, unambiguous detection required the development of a highly sensitive immunoblot procedure.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-2409297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-2418501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-2447293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-2878431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-3608154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-3941437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-3961490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-399327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-4765400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-6086950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-6180831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-6195530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-6356979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-6495660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-6699938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460642-7086958
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4786-90
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2460642-3,3'-Diaminobenzidine,
pubmed-meshheading:2460642-Benzidines,
pubmed-meshheading:2460642-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2460642-Gene Expression Regulation,
pubmed-meshheading:2460642-Genetic Vectors,
pubmed-meshheading:2460642-Hepatitis B virus,
pubmed-meshheading:2460642-Humans,
pubmed-meshheading:2460642-Immunoblotting,
pubmed-meshheading:2460642-Peptides,
pubmed-meshheading:2460642-RNA-Directed DNA Polymerase,
pubmed-meshheading:2460642-Virion
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pubmed:year |
1988
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pubmed:articleTitle |
Hepatitis B virus particles contain a polypeptide encoded by the largest open reading frame: a putative reverse transcriptase.
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pubmed:affiliation |
Department of Infectious Diseases, Cetus Corporation, Emeryville, California 94608.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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