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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1988-12-21
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pubmed:abstractText |
The proteins of viral envelope, encoded by the pre-S1 region of HBV-DNA, were measured quantitatively with enzyme immunoassay using monoclonal antibodies directed to pre-S1 epitope and correlated with the expression of pre-S2 region encoded epitope and other HBV markers. In acute HBV infection, both pre-S encoded proteins were detected in sera along with markers of viral replication and disappeared shortly before complete virus clearance while high HBsAg titers were still present. Pre-S1 antigen was present in most (95.5%) symptomatic and asymptomatic chronic HBsAg carriers. There was no correlation between the presence of pre-S1 and HBeAg or HBV-DNA in serum: 73% of sera with pre-S1 determinants were anti-HBe positive, and only 25.4% were positive for HBV-DNA. Most pre-S1 activity in sera of viremic carriers was detected in fractions of sucrose gradient containing subviral 22-nm particles, and much less in those containing infectious virions. In asymptomatic, nonviremic HBsAg carriers, pre-S1 was located only on subviral 22-nm forms. Pre-S1 positive particles had no accessible pre-S2 epitope, which is recognized specifically by monoclonal anti-pre-S2 (F124) antibody. These results show that the synthesis of the large protein of HBV envelope may occur also in the absence of active viral replication, and in these cases pre-S1 encoded sequences are on subviral particles of HBsAg. Therefore, pre-S1 is not a serologic marker of infectious virus. Disappearance of pre-S1 epitopes on HBsAg occurs only before complete clearance of the virus, and this may have potential prognostic relevance.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Surface Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0146-6615
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
217-25
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2460586-Acute Disease,
pubmed-meshheading:2460586-Carrier State,
pubmed-meshheading:2460586-Centrifugation, Density Gradient,
pubmed-meshheading:2460586-Chronic Disease,
pubmed-meshheading:2460586-DNA, Viral,
pubmed-meshheading:2460586-Epitopes,
pubmed-meshheading:2460586-Hepatitis B,
pubmed-meshheading:2460586-Hepatitis B Antigens,
pubmed-meshheading:2460586-Hepatitis B Surface Antigens,
pubmed-meshheading:2460586-Hepatitis B virus,
pubmed-meshheading:2460586-Humans,
pubmed-meshheading:2460586-Immunoenzyme Techniques,
pubmed-meshheading:2460586-Microscopy, Electron,
pubmed-meshheading:2460586-Prognosis,
pubmed-meshheading:2460586-Radioimmunoassay,
pubmed-meshheading:2460586-Viral Envelope Proteins,
pubmed-meshheading:2460586-Viremia,
pubmed-meshheading:2460586-Virion
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pubmed:year |
1988
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pubmed:articleTitle |
Occurrence of pre-S1 antigen in viremic and nonviremic carriers of hepatitis B surface antigen.
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pubmed:affiliation |
Microbial Immunology Unit, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article
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