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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1988-11-8
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pubmed:abstractText |
Campylobacter pylori has been implicated in the pathogenesis of peptide ulcer disease. The rapid identification of this organism may depend upon histologic diagnosis, because culture methods are complex and require a minimum of seven days in order to identify a negative specimen. The purpose of this study was to determine which stain used to identify this organism was the most cost-effective and easiest to perform and interpret on a routine basis. Sixty-one consecutive gastric antral biopsies were stained with hematoxylin and eosin, Giemsa, Brown-Brenn, and Warthin-Starry, with 23 of the cases stained by Brown-Hopps. Of the stains tested, the Wright-Giemsa was the easiest to perform. The organisms on the Wright-Giemsa showed a smooth, uniform purple color, whereas the Warthin-Starry gave the organism a granular appearance that at times could be confused for silver precipitate. Both the Wright-Giemsa and Brown-Hopps stain had the highest degree of identification of the organism (defined by percent positivity). The routine use of the Wright-Giemsa stain for identification of C. pylori in antral biopsies is recommended.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0002-9173
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
450-3
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2459956-Acute Disease,
pubmed-meshheading:2459956-Campylobacter,
pubmed-meshheading:2459956-Campylobacter Infections,
pubmed-meshheading:2459956-Chronic Disease,
pubmed-meshheading:2459956-Eosine Yellowish-(YS),
pubmed-meshheading:2459956-Gastritis,
pubmed-meshheading:2459956-Hematoxylin,
pubmed-meshheading:2459956-Humans,
pubmed-meshheading:2459956-Intestinal Mucosa,
pubmed-meshheading:2459956-Male,
pubmed-meshheading:2459956-Pyloric Antrum,
pubmed-meshheading:2459956-Staining and Labeling
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pubmed:year |
1988
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pubmed:articleTitle |
Evaluation of staining methods for identifying Campylobacter pylori.
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pubmed:affiliation |
Marshall University School of Medicine, Department of Pathology, Huntington, West Virginia 25702.
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pubmed:publicationType |
Journal Article,
Comparative Study
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