Linked Life Data

2458955

Source:http://linkedlifedata.com/resource/pubmed/id/2458955

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1988-11-2
pubmed:abstractText
Myeloid and erythroid progenitor cells were enriched from human marrow by selecting CD34-positive (CD34 + ve) cells, labeled with the My10 (HPCA-1) antibody, using a fluorescence-activated cell sorter. Seventy-one percent of CD34 + ve cells were blasts and most of these were too primitive to be identified by standard morphological criteria. On average, 9.5% of CD34 + ve cells formed clones after 14 days of culture in semisolid medium supplemented with erythropoietin and medium conditioned by 5637 bladder carcinoma cells. Over 2.5% of CD34 + ve cells were day-14 myeloid colony-forming cells and 2.4% were erythroid colony (burst)-forming progenitors. The remaining progenitors formed myeloid and erythroid clusters. A subpopulation of day-14 myeloid colony-forming cells failed to respond to recombinant human granulocyte-macrophage colony-stimulating factor (rhuGM-CSF) after accessory cells were removed during enrichment, so it appears that this factor can induce myeloid growth indirectly as well as directly. Recombinant human GM-CSF also supported erythroid colony-formation in cultures of CD34 + ve cells, which suggests that this hemopoietin may act directly on erythroid progenitors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
785-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:2458955-Antigens, CD34, pubmed-meshheading:2458955-Antigens, Differentiation, pubmed-meshheading:2458955-Bone Marrow Cells, pubmed-meshheading:2458955-Cell Aggregation, pubmed-meshheading:2458955-Cell Division, pubmed-meshheading:2458955-Cell Separation, pubmed-meshheading:2458955-Colony-Forming Units Assay, pubmed-meshheading:2458955-Colony-Stimulating Factors, pubmed-meshheading:2458955-Culture Media, pubmed-meshheading:2458955-Erythroblasts, pubmed-meshheading:2458955-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:2458955-Growth Substances, pubmed-meshheading:2458955-Hematopoiesis, pubmed-meshheading:2458955-Hematopoietic Stem Cells, pubmed-meshheading:2458955-Humans, pubmed-meshheading:2458955-Phenotype, pubmed-meshheading:2458955-Recombinant Proteins
pubmed:year
1988
pubmed:articleTitle
Enrichment of CD34 (My10)-positive myeloid and erythroid progenitors from human marrow and their growth in cultures supplemented with recombinant human granulocyte-macrophage colony-stimulating factor.
pubmed:affiliation
Department of Haematology, University Hospital, Wales, Cardiff, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't