Linked Life Data

2458863

Source:http://linkedlifedata.com/resource/pubmed/id/2458863

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1988-11-22
pubmed:abstractText
Any exploration of the physiology of secretion of free alpha subunit of glycoprotein hormones in humans requires a sensitive assay system that shows low cross reactivity with the intact hormones (lutropin, follitropin, thyrotropin, and choriogonadotropin). We established and validated such an assay system with a monoclonal antibody for detection of free alpha subunit in serum with better sensitivity (detection limit, 30 ng/L) and less cross reactivity (0.67% with intact human lutropin) than with available polyclonal antibody methods. Thus, the specific epitope recognized by this antibody apparently is exposed only on the free or uncombined form of alpha subunit and is concealed after non-covalent combination with any beta subunit. Using Western blot analysis, we show that most of the cross reactivity is probably the result of contamination of the human lutropin standard (obtained from the National Hormone and Pituitary Program, NIH) with small amounts of free alpha subunit. With such a specific and sensitive method, it should be possible to obtain more-precise information regarding the secretion of free alpha subunit.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0009-9147
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2022-5
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Improved measurement of free alpha subunit of glycoprotein hormones by assay with use of a monoclonal antibody.
pubmed:affiliation
Reproductive Endocrine Unit, Massachusetts General Hospital, Boston 02114.
pubmed:publicationType
Journal Article