Linked Life Data

2458331

Source:http://linkedlifedata.com/resource/pubmed/id/2458331

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-10-24
pubmed:abstractText
In the present report we describe the isolation and characterization of stable, long-term, human T-lymphocyte clones specific for myelin basic protein (MBP) from multiple sclerosis (MS) patients. Isolation of these clones appeared possible only by seeding peripheral blood mononuclear cells into a limiting dilution microculture system containing MBP, autologous irradiated cells and Interleukin-2 (IL-2), thereby minimizing effects of putative suppressor cell populations. All clones obtained were of the CD4+ phenotype. The majority was capable of MBP-specific cytolysis, tested with 51Chromium-labeled autologous Epstein-Barr virus (EBV)-transformed B-cells, coated with MBP, as targets. A few other clones had natural killer (NK) function. All clones produced Interleukin-2 (IL-2) upon adequate stimulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0198-8859
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-109
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Myelin basic protein-specific CD4+ cytolytic T-lymphocyte clones isolated from multiple sclerosis patients.
pubmed:affiliation
Department of Neurology, Academic Hospital Maastricht, University of Limburg, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't