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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-10-19
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pubmed:abstractText |
The Philadelphia (Ph1) chromosome in chronic myelogenous leukemia (CML) involves reciprocal translocation of the bcr gene and the c-abl oncogene. The fused bcr/abl gene is transcribed into two types of chimeric mRNA. By means of a combined method of S1 nuclease protection and polymerase chain reaction, we amplified sequences representative of the chimeric bcr/abl transcripts. Only 5 micrograms of total cellular RNA is needed and the chimeric bcr/abl message can be detected at a dilution of 1:100,000. We also detected residual chimeric bcr/abl transcripts in the remission samples from two Ph1-positive CML patients. This technique has the potential to identify a subpopulation of Ph1-positive CML patients in remission who are at high risk of relapse.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
893-7
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2458151-Base Sequence,
pubmed-meshheading:2458151-DNA-Directed DNA Polymerase,
pubmed-meshheading:2458151-Fusion Proteins, bcr-abl,
pubmed-meshheading:2458151-Humans,
pubmed-meshheading:2458151-Leukemia, Myeloid,
pubmed-meshheading:2458151-Molecular Sequence Data,
pubmed-meshheading:2458151-Neoplasm Proteins,
pubmed-meshheading:2458151-Nucleic Acid Hybridization,
pubmed-meshheading:2458151-Philadelphia Chromosome,
pubmed-meshheading:2458151-Proto-Oncogene Proteins,
pubmed-meshheading:2458151-RNA,
pubmed-meshheading:2458151-Transcription, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
Detection of minimal residual bcr/abl transcripts by a modified polymerase chain reaction.
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pubmed:affiliation |
Division of Laboratory Medicine, University of Texas System Cancer Center, Houston 77030.
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pubmed:publicationType |
Journal Article
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