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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-10-7
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pubmed:abstractText |
The isolated activation segment of pig procarboxypeptidase A binds two Tb3+ ions in a strong and specific way. In contrast, the binding of Ca2+, Cd2+ and Mg2+ is weak. The binding of Tb3+ increases the resistance of the isolated activation segment against proteolysis and competes for the binding of the carbocyanine dye Stains-All. This dye forms complexes with the activation segment showing spectral properties similar to those observed with EF-hand structures. The presented results support a previous hypothesis on the existence of two regions in the activation segment of pancreatic procarboxypeptidases structurally related to Ca2+-binding domains of the EF-hand protein family.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-1238120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-163769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-2427135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-2933134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-293720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-3426597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-3792135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-3801014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-3902502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-3997808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-3999139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-4004793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-4052013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-4052373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-4425648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-5079891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-5529813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-558800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6105043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6190419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6193121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6275388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6276400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6369538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6432301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6469946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6626179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6653776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-6753834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-7138880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-7152041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-7272283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2458099-836870
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
251
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
901-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2458099-Animals,
pubmed-meshheading:2458099-Binding Sites,
pubmed-meshheading:2458099-Calcium,
pubmed-meshheading:2458099-Carbocyanines,
pubmed-meshheading:2458099-Carboxypeptidases,
pubmed-meshheading:2458099-Carboxypeptidases A,
pubmed-meshheading:2458099-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2458099-Enzyme Activation,
pubmed-meshheading:2458099-Enzyme Precursors,
pubmed-meshheading:2458099-Ligands,
pubmed-meshheading:2458099-Macromolecular Substances,
pubmed-meshheading:2458099-Protein Conformation,
pubmed-meshheading:2458099-Spectrophotometry,
pubmed-meshheading:2458099-Staining and Labeling,
pubmed-meshheading:2458099-Swine,
pubmed-meshheading:2458099-Terbium,
pubmed-meshheading:2458099-Trypsin
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pubmed:year |
1988
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pubmed:articleTitle |
Analysis of the conformation and ligand-binding properties of the activation segment of pig procarboxypeptidase A.
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pubmed:affiliation |
Departament de Bioquimica i Biologia Molecular (Unitat de Ciències), Universitat Autònoma de Barcelona, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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