Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-9-2
pubmed:abstractText
Adherent PMNLs secrete protein factor termed granulocyte factor (GF). The antigenic and some structural features of GF were examined in the present paper. Molecular weight of GF was established on Sephadex G-75 column and is amounted to 40 kD. GF exhibited two isoelectric points in pH ranged from 4.7 to 4.9 and from 5.7 to 6.4. SDS polyacrylamide gel electrophoresis has displayed two bands, 20 and 68 kD, respectively. Initial analysis of amino acid composition of purified GF has shown 15 amino acids, but without aromatic and thioamino acids. Antigen-dependent species specificity was confirmed using rabbit anti-human, -murine, -guinea pig or-rat - GF IgG applying conventional precipitation methods in agar gel no crossreaction was noted. Using precipitation analyses GF was localized in the specific granules of PMNLs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0323-4398
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
The immunoregulatory granulocyte factor (GF). I. Some antigenic and structure features.
pubmed:affiliation
Department of Pathophysiology, Institute of Basic Medical Sciences WAM, Lód?.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't