rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
3
|
pubmed:dateCreated |
1988-8-25
|
pubmed:abstractText |
Recombinant human granulocyte-colony-stimulating factor (G-CSF) was purified from Chinese hamster ovary cells transfected with human G-CSF cDNA. The recombinant human G-CSF was treated with alkaline borohydride and the oligosaccharide-alditols liberated were fractioned by gel filtration on a Bio-Gel P-4 column, followed by high-performance liquid chromatography by use of a strong anion exchanger. Two oligosaccharide-alditols were obtained and their structures were identified by component analysis and 500-MHz 1H-NMR spectroscopy. The structures of the sugar chains were NeuAc alpha 2-3Gal beta 1-3GalNAcol and NeuAc alpha 2-3Gal beta 1-3(NeuAc alpha 2-6)GalNAcol.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0021-924X
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
103
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
544-6
|
pubmed:dateRevised |
2007-12-19
|
pubmed:meshHeading |
pubmed-meshheading:2455710-Animals,
pubmed-meshheading:2455710-Carbohydrates,
pubmed-meshheading:2455710-Chemical Fractionation,
pubmed-meshheading:2455710-Colony-Stimulating Factors,
pubmed-meshheading:2455710-Cricetinae,
pubmed-meshheading:2455710-Cricetulus,
pubmed-meshheading:2455710-Female,
pubmed-meshheading:2455710-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:2455710-Granulocytes,
pubmed-meshheading:2455710-Magnetic Resonance Spectroscopy,
pubmed-meshheading:2455710-Oligosaccharides,
pubmed-meshheading:2455710-Ovary,
pubmed-meshheading:2455710-Recombinant Proteins
|
pubmed:year |
1988
|
pubmed:articleTitle |
Structures of the sugar chains of recombinant human granulocyte-colony-stimulating factor produced by Chinese hamster ovary cells.
|
pubmed:affiliation |
Department of Chemistry, Osaka University College of Science.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|