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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1988-8-2
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pubmed:abstractText |
The effect of interferon (IFN) treatment on the early stages of herpes simplex virus type 1 (HSV-1) replication in three types of human cells was investigated. Interferon pretreatment was shown to reduce the steady state levels of both total and polysomebound HSV-1 immediate early alpha mRNAs. Using the nuclear run-off transcription assay, we showed that IFN selectively inhibited transcription of the HSV-1 genes, with no effect on transcription of total cellular RNA or that of the beta-tubulin RNA. Thus, IFN appears to inhibit the initiation of HSV-1 alpha gene transcription rather than affect the stability of the respective mRNAs. IFN did not prevent the HSV-1-induced early shut-off of host cellular protein synthesis caused by a structural protein of infecting virus. This observation indicated that the IFN-mediated inhibition of HSV-1 replication is at a stage beyond viral penetration into the cytoplasm. These results suggested that IFN blocked HSV-1 replication primarily at a very early stage, during the onset of alpha mRNA transcription.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/IE1 protein, Human herpesvirus 1,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferons,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69 ( Pt 6)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1167-77
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2455018-Cell Line,
pubmed-meshheading:2455018-DNA-Directed DNA Polymerase,
pubmed-meshheading:2455018-Encephalomyocarditis virus,
pubmed-meshheading:2455018-Fibroblasts,
pubmed-meshheading:2455018-Gene Expression Regulation,
pubmed-meshheading:2455018-Genes, Viral,
pubmed-meshheading:2455018-HeLa Cells,
pubmed-meshheading:2455018-Humans,
pubmed-meshheading:2455018-Immediate-Early Proteins,
pubmed-meshheading:2455018-Interferons,
pubmed-meshheading:2455018-Kinetics,
pubmed-meshheading:2455018-Nucleic Acid Hybridization,
pubmed-meshheading:2455018-RNA, Messenger,
pubmed-meshheading:2455018-RNA, Viral,
pubmed-meshheading:2455018-Recombinant Proteins,
pubmed-meshheading:2455018-Simplexvirus,
pubmed-meshheading:2455018-Transcription, Genetic,
pubmed-meshheading:2455018-Viral Proteins,
pubmed-meshheading:2455018-Virus Replication
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pubmed:year |
1988
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pubmed:articleTitle |
Inhibition of transcription of herpes simplex virus immediate early genes in interferon-treated human cells.
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pubmed:affiliation |
Department of Virology, Hebrew University Hadassah Medical School, Jerusalem, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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