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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1988-8-3
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pubmed:abstractText |
Advances in our understanding of the structure and molecular biology of the T lymphocyte antigen-receptor have now made it feasible to study human autoimmune diseases using new approaches. One such approach involves cloning of T cells from sites of autoimmune pathology followed by identification of putative disease-related T cell oligoclonality at the level of the T cell receptor gene rearrangements. We have now tested the feasibility of this approach in an animal model of autoimmunity, murine experimental allergic encephalomyelitis (EAE). Spinal cord-derived, self (murine) myelin basic protein (MBP)-reactive T cell lines and sublines were analyzed at the level of their receptor beta chain rearrangements using Southern blots. We now report that the MBP-reactive T cell lines and sublines derived from the spinal cords of four of five SJL/J mice with EAE share a 14.5-kb rearranged T cell receptor beta 1 band on Southern blots. A spinal cord-derived T cell line that was reactive to purified protein derivative of tuberculin (PPD), several lymph node-derived ovalbumin- and PPD-reactive T cell lines, as well as one MBP-reactive spinal cord-derived T cell line did not share this 14.5-kb rearranged beta 1 band. These results suggest that analysis of the antigen receptors used by T cells cloned from sites of inflammation may be a useful initial approach for identifying pathogenetically relevant T cells in the study of certain human autoimmune diseases.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-147420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-2413363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-2427582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-2579095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-2579139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-2579977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-291941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-3012351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-3029266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-3156277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-3456168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-3493439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-363224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-3874032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-4271082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-6231113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454949-6333305
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1810-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2454949-Animals,
pubmed-meshheading:2454949-Autoimmune Diseases,
pubmed-meshheading:2454949-Autoradiography,
pubmed-meshheading:2454949-Cell Line,
pubmed-meshheading:2454949-Cloning, Molecular,
pubmed-meshheading:2454949-DNA,
pubmed-meshheading:2454949-DNA Restriction Enzymes,
pubmed-meshheading:2454949-Deoxyribonuclease HindIII,
pubmed-meshheading:2454949-Disease Models, Animal,
pubmed-meshheading:2454949-Encephalomyelitis, Autoimmune, Experimental,
pubmed-meshheading:2454949-Lymphocyte Activation,
pubmed-meshheading:2454949-Male,
pubmed-meshheading:2454949-Mice,
pubmed-meshheading:2454949-Myelin Basic Proteins,
pubmed-meshheading:2454949-Nucleic Acid Hybridization,
pubmed-meshheading:2454949-Ovalbumin,
pubmed-meshheading:2454949-Receptors, Antigen, T-Cell,
pubmed-meshheading:2454949-Spinal Cord,
pubmed-meshheading:2454949-T-Lymphocytes,
pubmed-meshheading:2454949-Tuberculin
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pubmed:year |
1988
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pubmed:articleTitle |
T cell receptor beta chain gene rearrangement shared by murine T cell lines derived from a site of autoimmune inflammation.
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pubmed:affiliation |
Department of Medicine, University of Connecticut School of Medicine, Farmington 06032.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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