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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1988-8-8
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pubmed:abstractText |
This study was designed to analyse further the idiotypic cross-reactivity between anti-Lol p I murine monoclonal antibodies of IgG isotype and basophil-bound human IgE antibodies from grass pollen-sensitive patients. It was also designed to determine the expression frequency of the idiotypes present on cell-bound IgE. Rabbit anti-idiotypic antisera were produced against idiotypes of three anti-Lol p I monoclonal antibodies (290A-167, 539A-6 and 348A-6) of different specificities. Basophils from 19 patients reacting to Lol p I allergen, as shown by positive skin test reactions and by the presence of serum-specific IgE antibodies (measured by RAST), were challenged with these rabbit anti-idiotypic antibodies and the histamine released was measured. Our data indicate that IgE-borne idiotypes were expressed as follows: (i) co-expression of the three idiotypes in 15% of patients; (ii) co-expression of two idiotypes in 21% of patients; and (iii) expression of a unique idiotype (290A-167) in 42% of patients. Among the three idiotypes, 290A-167 was shown to be a public idiotype since it was expressed in 80% of patients. Fab fragments of anti-idiotypic antibodies could inhibit anti-idiotype-induced histamine release, but optimal conditions varied from one patient to another.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-14434282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-14446178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-2422284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-2439450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-4208922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-6207231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-6402708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-69001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-701806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-7381201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-80953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2454883-82603
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2454883-Adult,
pubmed-meshheading:2454883-Antibodies, Monoclonal,
pubmed-meshheading:2454883-Basophils,
pubmed-meshheading:2454883-Cross Reactions,
pubmed-meshheading:2454883-Dose-Response Relationship, Immunologic,
pubmed-meshheading:2454883-Histamine Release,
pubmed-meshheading:2454883-Humans,
pubmed-meshheading:2454883-Immunoglobulin E,
pubmed-meshheading:2454883-Immunoglobulin Fab Fragments,
pubmed-meshheading:2454883-Immunoglobulin G,
pubmed-meshheading:2454883-Immunoglobulin Idiotypes,
pubmed-meshheading:2454883-Middle Aged,
pubmed-meshheading:2454883-Rhinitis, Allergic, Seasonal
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pubmed:year |
1988
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pubmed:articleTitle |
Demonstration of idiotypes expressed on basophil-bound IgE antibodies by using anti-idiotype-induced histamine release in grass pollen-allergic patients.
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pubmed:affiliation |
Unité d'Immuno-Allergie, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article
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