Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1988-7-25
|
| pubmed:abstractText |
The whole molecule of human somatomedin C (SM-C) prepared by the total synthesis method was used as an antigen to produce an antiserum for a radioimmunoassay. Since plasma proteins that bind SM-C interfere with the assay, a method was developed that uses acid dissociation followed by C-2 cartridge extraction to strip SM-C from its binding proteins before assay. This assay has no cross-reactivity with human proinsulin or insulin-like growth factor II (IGF-II). The SM-C values in 339 normal subjects showed age-dependence, increasing from childhood to a peak at age 14 to 16 years and decreasing sharply before adulthood. In adults, the SM-C values decreased gradually with age. All 13 patients with acromegaly who were tested had an increased SM-C value, with no overlap with the normal range. The 12 patients with prolactinoma but non-growth-hormone-producing pituitary tumor had no increase in SM-C. Two children with pituitary deficiency had low SM-C values; one of these children received growth hormone therapy, and his SM-C value increased from undetectable to normal. By three weeks after discontinuation of the therapy, his SM-C value was again undetectable. Of 20 children with short stature and constitutional delay of growth and development, SM-C was below normal in 70 percent and normal in 30 percent. Two patients with malnutrition had below-normal SM-C values.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Proinsulin,
http://linkedlifedata.com/resource/pubmed/chemical/Somatomedins
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0091-7370
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
120-30
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2454603-Adolescent,
pubmed-meshheading:2454603-Adult,
pubmed-meshheading:2454603-Age Factors,
pubmed-meshheading:2454603-Carrier Proteins,
pubmed-meshheading:2454603-Child,
pubmed-meshheading:2454603-Child, Preschool,
pubmed-meshheading:2454603-Chromatography, Gel,
pubmed-meshheading:2454603-Cross Reactions,
pubmed-meshheading:2454603-Female,
pubmed-meshheading:2454603-Humans,
pubmed-meshheading:2454603-Hydrogen-Ion Concentration,
pubmed-meshheading:2454603-Immune Sera,
pubmed-meshheading:2454603-Infant,
pubmed-meshheading:2454603-Insulin-Like Growth Factor Binding Proteins,
pubmed-meshheading:2454603-Insulin-Like Growth Factor I,
pubmed-meshheading:2454603-Male,
pubmed-meshheading:2454603-Middle Aged,
pubmed-meshheading:2454603-Proinsulin,
pubmed-meshheading:2454603-Protein Binding,
pubmed-meshheading:2454603-Radioimmunoassay,
pubmed-meshheading:2454603-Somatomedins
|
| pubmed:articleTitle |
Assay of somatomedin C by cartridge extraction prior to radioimmunoassay with antiserum developed against synthetic somatomedin C.
|
| pubmed:affiliation |
Section of Clinical Chemistry, Mayo Clinic, Rochester, MN 55905.
|
| pubmed:publicationType |
Journal Article
|