|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
11
|
|
pubmed:dateCreated |
1988-7-8
|
|
pubmed:abstractText |
A methodology was developed for stable gene transfer into cloned nontransformed human T lymphocytes. Stable high-level gene expression was achieved in cloned human T cells by using a self-replicating Epstein-Barr virus (EBV) episomal replicon. A comparison of five eukaryotic promoters established that the Rous sarcoma virus 3' long terminal repeat (RSV 3' LTR) and the lymphopapilloma virus (LPV) 5' LTR are optimal for episome-based expression in T cells. Effective (greater than 95%), selective, and reversible anti-sense RNA-mediated gene inhibition of a model T-cell-associated molecule (CD8) was achieved in a cytotoxic human T-cell clone by using an EBV episome-based, RSV 3' LTR-driven expression system. The linking of anti-sense RNA mutagenesis and T-cell cloning technologies should contribute significantly to studies of human T-cell function.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-2418145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-2421164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-2427015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-2983224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-2985281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-2989696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3005404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3006253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3011901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3015413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3016899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3025615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3031507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3081815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3094962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3466022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3491296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3501369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3871356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-3929069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-6206143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-6236884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-6242467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-6294651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-6300662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-6350818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2453884-6960240
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
85
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4010-4
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2453884-Antigens, CD8,
pubmed-meshheading:2453884-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2453884-Cloning, Molecular,
pubmed-meshheading:2453884-Gene Expression Regulation,
pubmed-meshheading:2453884-Genetic Vectors,
pubmed-meshheading:2453884-Herpesvirus 4, Human,
pubmed-meshheading:2453884-Plasmids,
pubmed-meshheading:2453884-RNA,
pubmed-meshheading:2453884-RNA, Complementary,
pubmed-meshheading:2453884-Replicon,
pubmed-meshheading:2453884-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:2453884-Transfection
|
|
pubmed:year |
1988
|
|
pubmed:articleTitle |
Use of an Epstein-Barr virus episomal replicon for anti-sense RNA-mediated gene inhibition in a human cytotoxic T-cell clone.
|
|
pubmed:affiliation |
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
