Linked Life Data

2453017

Source:http://linkedlifedata.com/resource/pubmed/id/2453017

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1988-6-14
pubmed:abstractText
Avian pp60c-src, a proto-oncogene product, has been expressed in the yeast, Saccharomyces cerevisiae. pp60c-src from yeast is 10- to 15-fold more active than pp60c-src from mammalian fibroblasts. Extensive phosphorylation of pp60c-src occurs at tyrosine 527 in fibroblasts and inhibits pp60c-src kinase activity. In contrast, pp60c-src from yeast has low levels of phosphate at tyrosine 527. This phosphate may be added by an autophosphorylation reaction that is detectable when immunoprecipitates containing pp60c-src from yeast are incubated with ATP in vitro. This reaction is minor when compared with autophosphorylation at the principal site, tyrosine 416. These data suggest that pp60c-src in fibroblasts is normally inhibited by trans-acting factors, that either directly catalyze phosphate transfer to tyrosine 527 or augment autophosphorylation at this site. Such factors would have the potential to regulate pp60c-src kinase activity. The low but detectable ability of pp60c-src to autophosphorylate at an inhibitory site raises the possibility that pp60c-src may also be auto-regulated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0890-6467
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
297-310
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:articleTitle
Avian pp60c-src is more active when expressed in yeast than in vertebrate fibroblasts.
pubmed:affiliation
Department of Cell Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.