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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1988-6-20
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pubmed:abstractText |
The epitopes of a panel of five monoclonal antibodies to the Sendai virus P protein were mapped by generating modified forms of the P mRNA via SP6 expression followed by in vitro translation. The epitopes were found to be clustered in the C-terminal region of the protein. Two epitopes were within the last 30 residues, two were within the next 65, and one was between residues 308 and 451 of this 568-residue-long protein. By a combination of partial proteolysis and Western immunoblotting with one of these antibodies, the sites at which phosphates are added in vitro by the virion-associated kinase were mapped to the second quarter of the molecule from the N terminus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-235597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-2578238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-2821189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-2834203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-2987524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-3018671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-320200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-4359304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-6183344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-6245261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-6285600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-6317203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2452902-6683907
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed RNA Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/P protein, Sendai virus,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2200-3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2452902-Antibodies, Monoclonal,
pubmed-meshheading:2452902-Antibodies, Viral,
pubmed-meshheading:2452902-Antigens, Viral,
pubmed-meshheading:2452902-Cloning, Molecular,
pubmed-meshheading:2452902-DNA-Directed RNA Polymerases,
pubmed-meshheading:2452902-Epitopes,
pubmed-meshheading:2452902-Molecular Weight,
pubmed-meshheading:2452902-Parainfluenza Virus 1, Human,
pubmed-meshheading:2452902-Phosphoproteins,
pubmed-meshheading:2452902-Phosphorylation,
pubmed-meshheading:2452902-Viral Proteins
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pubmed:year |
1988
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pubmed:articleTitle |
Mapping of monoclonal antibodies to the Sendai virus P protein and the location of its phosphates.
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pubmed:affiliation |
Department of Microbiology, Geneva Medical School, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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