Linked Life Data

2452204

Source:http://linkedlifedata.com/resource/pubmed/id/2452204

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-5-27
pubmed:abstractText
Phosphotyrosine coupled to KLH, BSA, and OVA was used for the production and screening of antibodies to phosphotyrosine. 800 hybridomas secreting antibodies that bound to phosphotyrosine were detected by ELISA. The most reactive 100 of these 800 were tested subsequently for their ability to bind phosphotyrosine-containing proteins on Western blots. Eight stable hybridoma cell lines were selected for further study, cloned by limiting dilution, and grown as ascites. These antibodies were purified by three different methods, and it was found that affinity chromatography on phosphotyrosine-affigel provided the most rapid and effective method. Many phosphotyrosine-containing proteins were detected by using these antibodies in Western blotting and immunoaffinity purification procedures. Binding of anti-phosphotyrosine antibody could be competed by phosphotyrosine or phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate. These antibodies should be useful for the identification and purification of proteins phosphorylated on tyrosine residues in transformed and growth factor-treated cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
277-85
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Monoclonal antibodies to phosphotyrosine.
pubmed:affiliation
Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92138.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.