Linked Life Data

2451678

Source:http://linkedlifedata.com/resource/pubmed/id/2451678

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-5-24
pubmed:abstractText
A sensitive and specific avidin-biotin enzyme immunoassay that uses immunoreagents from naturally infected individuals was formulated for the detection of human immunodeficiency virus (HIV) antigens. A total of 500 cell culture samples from 111 cultures were tested in this assay and in a reverse transcriptase (RT) assay. Of 353 samples that were positive in the immunoassay, 174 were positive and 179 were negative in the RT assay. The specificity of the immunoassay results was supported by the failure of samples to react with nonimmune serum, by the ability of an anti-HIV type 1 monoclonal antibody to block the reactivity of selected samples, and by the appearance of RT activity in samples drawn from some cultures after a longer period of cultivation. HIV antigens were detected in 174 of 176 RT-positive samples (sensitivity, 98.9%). A comparison of the kinetics of antigen production and RT activity revealed that detectable antigen levels frequently preceded the appearance of RT activity. Thus, 50% of virus-containing cultures were identified within 9 days by immunoassay compared with 14 days by RT assay. In addition, RT activity was often detected intermittently in cultures sampled on several days, whereas antigen levels did not decline after initial appearance. Enzyme immunoassays for HIV antigen detection that use easily obtained reagents and simple technologies could facilitate laboratory and clinical research which requires cultivation of viruses.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2413137, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2423552, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2428827, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2437321, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2439537, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2861512, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2873436, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2878129, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2981931, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-2989707, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-3025743, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-6189183, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-6200936, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-6206563, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-6268655, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-6803327, http://linkedlifedata.com/resource/pubmed/commentcorrection/2451678-90074
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
453-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Enzyme immunoassay for detection of human immunodeficiency virus antigens in cell cultures.
pubmed:affiliation
Department of Medicine and Pediatrics, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.