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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1988-5-3
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pubmed:abstractText |
The objective of this study was to analyze surface properties of larval Dirofilaria immitis with potential relevance to protective immunity. Comparisons were made between third (L3)- and fourth-stage larvae (L4) based on their net surface charge, surface carbohydrate and antigen composition, ability to nonspecifically absorb host proteins, complement activation, and nonspecific cellular adherence. It was determined that L3 had a net negative surface charge, whereas L4 had either a neutral or weakly positive surface charge. The lectin Con A, but not any of the other lectins tested, bound only to the surface of L4, and not to that of L3. Monoclonal antibodies were prepared which reacted with the surface of L3 or with the surface of L4, but never both. L4 were found to nonspecifically adsorb host protein to their surfaces, whereas L3 did not. Both L3 and L4 were found to activate complement through the alternate pathway. Finally, nonspecific cellular adherence was found on L3 both in vitro and in vivo but not on L4. The surfaces of L3 and L4 were thus shown to be significantly different and, potentially, in ways which would have great impact in the generation and effectiveness of a protective immune response.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Helminth,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Carbohydrates,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-4894
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
157-67
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2450771-Animals,
pubmed-meshheading:2450771-Antibodies, Monoclonal,
pubmed-meshheading:2450771-Antigens, Helminth,
pubmed-meshheading:2450771-Antigens, Surface,
pubmed-meshheading:2450771-Carbohydrates,
pubmed-meshheading:2450771-Cell Adhesion,
pubmed-meshheading:2450771-Complement Activation,
pubmed-meshheading:2450771-Complement Pathway, Alternative,
pubmed-meshheading:2450771-Dirofilaria immitis,
pubmed-meshheading:2450771-Epitopes,
pubmed-meshheading:2450771-Filarioidea,
pubmed-meshheading:2450771-Immunoglobulins,
pubmed-meshheading:2450771-Larva,
pubmed-meshheading:2450771-Lectins,
pubmed-meshheading:2450771-Mice,
pubmed-meshheading:2450771-Microscopy, Electron,
pubmed-meshheading:2450771-Serum Albumin,
pubmed-meshheading:2450771-Surface Properties
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pubmed:year |
1988
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pubmed:articleTitle |
Dirofilaria immitis: surface properties of third- and fourth-stage larvae.
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pubmed:affiliation |
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.
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pubmed:publicationType |
Journal Article,
Comparative Study
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