Linked Life Data

2450083

Source:http://linkedlifedata.com/resource/pubmed/id/2450083

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-4-19
pubmed:abstractText
The development and characteristics of rat alveolar type II cells were monitored by using various cytochemical techniques. Polarized light microscopy was found useful for observing live type II cells in culture. Cells progressively lose their birefringent granules starting from 48 h of cells in culture, indicating the disappearance of the phospholipids organized lamellae in the lamellar bodies. Similar results were obtained by using an immunocytochemical approach with antibodies raised against the apoprotein component of rat surfactant. A progressive decrease in immuno-staining corresponded to the disappearance of the lamellar bodies, and birefringence. Changes in lectins binding to the cultured type II cells were also observed. Freshly isolated and one day old cultured cells could bind Macula pomifera (MPA) but not Arachis hypogaea (PA) lectins. The reverse was found in 6-7 days old cultured cells which had the ability to bind PA but not MPA the advantage of using various cytochemical techniques for studying the development of type II cells in culture is being discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5564
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-9
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Cytochemical approach to the development and behaviour of rat alveolar type II cells in culture.
pubmed:affiliation
Department of Histology and Cell Biology, Sackler School of Medicine, Tel Aviv University, Israel.
pubmed:publicationType
Journal Article