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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1988-3-29
|
| pubmed:abstractText |
Transfer factor specific for herpes simplex virus (HSV) type 1 (TFHSV-1) was prepared from splenic cells of HSV-1 immunized mice. Protection was transferred with TFHSV-1 to nonimmune mouse recipients. The TFHSV-1 injected mice had a higher survival rate after lethal HSV-1 challenge as compared to mice injected with a nonspecific transfer factor (P less than 0.05). 51Cr-labelled leukocyte adherence inhibition (51-Cr-LAI) test was used to demonstrate the specific activity of transfer factor in vitro. Only leukocytes incubated with TFHSV-1 exhibited significant adherence inhibition (P less than 0.01) to HSV-1 antigen, but not to control antigen. Specific activity component of TFHSV-1 (STFc) was separated by affinity adsorption with the antigen. Activity of STFc in 51Cr-LAI test was significantly higher than that of TFHSV-1 (P less than 0.01). Ratio activity of STFc in protective host immunity was 16 times as much as that of TFHSV-1. STFc was analysed by high performance liquid chromatography, thin layer chromatography and isoelectric focusing in the polyacrylamide gel. Results revealed that STFc appeared to be a polypeptide with a molecular weight of about 12,870 dalton.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0001-723X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
31
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
449-57
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2449810-Amino Acids,
pubmed-meshheading:2449810-Animals,
pubmed-meshheading:2449810-Antigens, Viral,
pubmed-meshheading:2449810-Chromatography, High Pressure Liquid,
pubmed-meshheading:2449810-Chromatography, Ion Exchange,
pubmed-meshheading:2449810-Chromatography, Thin Layer,
pubmed-meshheading:2449810-Epitopes,
pubmed-meshheading:2449810-Herpes Simplex,
pubmed-meshheading:2449810-Immunity, Cellular,
pubmed-meshheading:2449810-Isoelectric Focusing,
pubmed-meshheading:2449810-Leukocyte Adherence Inhibition Test,
pubmed-meshheading:2449810-Leukocytes,
pubmed-meshheading:2449810-Male,
pubmed-meshheading:2449810-Mice,
pubmed-meshheading:2449810-Mice, Inbred BALB C,
pubmed-meshheading:2449810-Molecular Weight,
pubmed-meshheading:2449810-Simplexvirus,
pubmed-meshheading:2449810-Transfer Factor
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Nature and antigen-specific activities of transfer factor against herpes simplex virus type 1.
|
| pubmed:affiliation |
Dpt. of biochemistry, Fourth Military Medical College, Xian, China.
|
| pubmed:publicationType |
Journal Article
|