Linked Life Data

2449797

Source:http://linkedlifedata.com/resource/pubmed/id/2449797

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1988-4-7
pubmed:abstractText
Antigen detection for diagnosis of pneumococcal infections has earlier been based on the use of antibodies against capsular antigens. Methods based on the demonstration of C-polysaccharide (PnC) have the advantage of using antibodies against one single species-specific antigen instead of applying a polyvalent mixture of antisera against 83 different capsular antigens. Very little has been done earlier to evaluate the accessibility of the PnC on the surface of pneumococcal cells, particularly cells carrying capsules, and the release of PnC to the environment. We have used a monoclonal anti-PnC antibody in an ELISA inhibition test to demonstrate PnC during growth from four different Streptococcus pneumoniae strains (capsular types 1, 3 and 19F) and a C-mutant strain, reported to carry PnC as a small capsule. Heavily-capsulated types 3 and 19F exposed more PnC on their surface than the type 1 strain, and considerable amounts of PnC were released to the culture medium during growth. The C-mutant strain differed from the other strains in that it exposed less PnC on its surface. The type 1 strain and the C-mutant released approximately the same amount of PnC to the culture medium. Treatment with antibiotics during growth caused a decrease in surface-located PnC but did not significantly affect the amount released. The total accessible PnC in these cultures was quite significant and well above the limit of detection in an ELISA earlier described for the detection of PnC in clinical samples. The results presented here give support to the notion that the demonstration of PnC in clinical samples should provide a good basis for diagnosis of pneumococcal pneumonia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0108-0180
pubmed:author
pubmed:issnType
Print
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
371-8
pubmed:dateRevised
2009-6-4
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Availability of reaction with antibodies of the pneumococcal C-polysaccharide on the surface of capsulated pneumococci.
pubmed:affiliation
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.
pubmed:publicationType
Journal Article