Linked Life Data

2449589

Source:http://linkedlifedata.com/resource/pubmed/id/2449589

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-4-4
pubmed:abstractText
A novel method to process nervous tissue for neuroanatomical studies is presented. In this cryostat method, the brain tissue is first treated with microwave irradiation after perfusion with physiological saline. Cutting quality of the microwave-treated brain tissue was excellent. The sections were stained with the Nissl staining, the Klüver-Barrera method, and the Bodian method, respectively. In addition, immunocytochemistry using the RAM/PO method was performed. For the latter a monoclonal antibody against neurofilament (NF90), recognizing the 200, 150, and 70 kD units was used. Excellent results were obtained. The advantage of the microwave-cryostat technique for processing of nervous tissue is not merely a substantial reduction of time to obtain microscopical slides of good quality. This method allows in addition the estimation of the effect of fixation by formaldehyde in immunocytochemistry.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-101
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Microwave-cryostat technique for neuroanatomical studies.
pubmed:affiliation
Laboratory for Anatomy and Embryology, University of Leiden, The Netherlands.
pubmed:publicationType
Journal Article