Linked Life Data

2449539

Source:http://linkedlifedata.com/resource/pubmed/id/2449539

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-3-28
pubmed:abstractText
The antibody recognition of synthetic rabbit encephalitogen S24 (TTHYGSLPQKG), which had been inactivated by extensive iodination, was fully restored by tritium-iodine exchange during boron hydride reduction. A highly active form of 3H-S24 was obtained by reverse-phase high-performance liquid chromatography (HPLC) purification of the crude product and could be further separated into three continuous subfractions, the latter two of which were fully active from an immunochemical point of view. A major epitope was disclosed through use of the 3H-S24 probe, which required the intact S24 peptide for its expression. Confirmation of two linear epitopes previously encountered, GSLPQK and THYGSL, was also obtained, the latter through the use of the third HPLC subfraction of 3H-S24.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
493-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
A major epitope of synthetic rabbit encephalitogen S24 disclosed through the use of a tritiated peptide probe.
pubmed:affiliation
Department of Microbiology-Immunology, Duke University Medical Center, Durham, NC 27710.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.