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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-4-7
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pubmed:abstractText |
Two fragments (21-mers) containing consensus sequences for the self-cleavage domain in transcripts of satellite DNA of the newt were chemically synthesized and found to be cleaved in the presence of Mg2+. The cleaved product contained the 3'-terminal 2',3'-cyclic phosphate. Twenty-five combinations of partially double-stranded 21-mer RNA which contained different bases within the consensus sequences and at the cleavage sites were tested for self-cleavage. It seemed that guanosine 3'-phosphate was not susceptible to transesterification at the cleavage site.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
228
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
228-30
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:2449365-Animals,
pubmed-meshheading:2449365-Base Composition,
pubmed-meshheading:2449365-Base Sequence,
pubmed-meshheading:2449365-DNA, Satellite,
pubmed-meshheading:2449365-Esterification,
pubmed-meshheading:2449365-Magnesium,
pubmed-meshheading:2449365-Polyribonucleotides,
pubmed-meshheading:2449365-RNA,
pubmed-meshheading:2449365-RNA, Double-Stranded,
pubmed-meshheading:2449365-Salamandridae,
pubmed-meshheading:2449365-Transcription, Genetic
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pubmed:year |
1988
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|
pubmed:articleTitle |
Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.
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pubmed:affiliation |
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
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pubmed:publicationType |
Journal Article
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