Linked Life Data

2448332

Source:http://linkedlifedata.com/resource/pubmed/id/2448332

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1988-3-21
pubmed:abstractText
Studies were designed to test the hypothesis that homologous proteins are expressed in elasmobranch scale, tooth enameloid, and mammalian enamel. Using indirect immunohistochemistry and high-resolution two-dimensional gel electrophoresis with immunoblotting, mouse enamel proteins were compared with placoid scale and enameloid proteins from the swell shark, Cephaloscyllium ventriosum. Swiss Webster mouse molar teeth show a characteristic enamel protein pattern consisting of two anionic enamel proteins of 72 kDa (pI 5.8) and 46 kDa (pI 5.5) and several more basic and lower-molecular-weight enamel polypeptides. Both anionic and basic classes of enamel proteins cross-reacted with either antiamelogenin or antienamelin antibodies. Placoid scale and tooth enameloid contained two anionic proteins identified as 58 kDa (pI 5.7) and 46 kDa (pI 5.5), which cross-reacted with either antimouse amelogenin or antihuman enamelin IgG antibodies. A minor antigenically related protein of 43 kDa (pI 6.2) was detected. Immunochemical staining showed localization within placoid scale, swell shark inner enamel epithelia, enameloid, and mouse inner enamel epithelia and enamel. We interpret these results to suggest that both placoid scale and enameloid proteins share epitopes and that these epitopes are also shared with mammalian enamel proteins. Based on molecular weights, isoelectric pH values, and amino acid compositions, placoid scale and enameloid ECM proteins do not contain amelogenin proteins. We suggest that enamelinlike proteins are highly conserved during vertebrate evolution and that these relatively anionic macromolecules may serve a primary function in the initiation of calcium hydroxyapatite formation during enameloid biomineralization.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0270-4145
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
371-86
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Immunochemical homology between elasmobranch scale and tooth extracellular matrix proteins in Cephaloscyllium ventriosum.
pubmed:affiliation
Graduate Program in Craniofacial Biology, School of Dentistry, University of Southern California, Los Angeles 90089-0191.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.