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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1988-2-23
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pubmed:abstractText |
End-labelled oligodeoxynucleotides were injected into Xenopus laevis oocytes and their degradation products were analysed by high-performance ion-exchange liquid chromatography after various times of incubation. The oligonucleotides were synthesised with either the natural [beta] anomers or the synthetic [alpha] anomers of deoxynucleotide units. Oligo-[beta] deoxynucleotides are short-lived inside oocytes (half-life approximately equal to 10 min). Covalent attachment of an intercalating agent to the 3'-phosphate and of a methylthiophosphate group at the 5'-end protects oligodeoxynucleotides against 3'- and 5'-exonucleases, respectively. The half-life of such substituted oligodeoxynucleotides is increased to 40 minutes. Oligo-[alpha]-deoxynucleotides are quite resistant to both endo and exonucleases inside Xenopus oocytes. After 8 hours only 40% of a 16-mer oligo-[alpha]-deoxynucleotide were hydrolysed. The rapid degradation of oligo-[beta]-deoxynucleotides suggests that efficient inhibition of translation in Xenopus oocytes involves an RNase H-induced hydrolysis of mRNAs hybridized to oligo-[beta]-deoxynucleotides.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-2418466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-2427015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-3002493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-3037483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-3096384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-3474645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-3575096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-3627982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-3667725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-3855537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-3875079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-6193395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-6587350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2447563-75546
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10507-21
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2447563-Acridines,
pubmed-meshheading:2447563-Animals,
pubmed-meshheading:2447563-Chromatography, High Pressure Liquid,
pubmed-meshheading:2447563-Microinjections,
pubmed-meshheading:2447563-Oligodeoxyribonucleotides,
pubmed-meshheading:2447563-Oocytes,
pubmed-meshheading:2447563-RNA,
pubmed-meshheading:2447563-RNA, Antisense,
pubmed-meshheading:2447563-Time Factors,
pubmed-meshheading:2447563-Xenopus laevis
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pubmed:year |
1987
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pubmed:articleTitle |
Rate of degradation of [alpha]- and [beta]-oligodeoxynucleotides in Xenopus oocytes. Implications for anti-messenger strategies.
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pubmed:affiliation |
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U.201, Paris, France.
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pubmed:publicationType |
Journal Article
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