Linked Life Data

2447492

Source:http://linkedlifedata.com/resource/pubmed/id/2447492

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1988-1-25
pubmed:abstractText
We have analyzed the expression of immunoglobulin lambda chains in the rat by hybridizing RNA from various sources with C lambda 1-like and C lambda 2-like sequences recovered from a rat genomic library. A 1.0 kb lambda 2-like sequence is readily detected in lambda-producing hybridomas and in normal rat spleen RNAs; a 1.0 kb lambda 1-like message is also present, although at much lower levels. An additional 700 b.p. C lambda 2-like fragment is found in all normal rat spleens, and presumably represents a defective message. The nucleotide sequence of one cDNA clone isolated from the lambda-producing hybridoma G36/1 shows a lambda 2-like sequence, and six lambda-secreting hybridomas produced from the spleen of a kappa-suppressed rat all express a C lambda 2-like message. The great majority of rat lambda chains therefore appear to be lambda 2-like. Northern blot analysis of RNA from the spleen of this kappa-suppressed rat shows a considerable increase in the expression of both lambda 2-like and (at lower levels) lambda 1-like message. The coordinate rise of lambda 1 and lambda 2 RNA in this rat suggests that there may be at least two functional lambda chain genes in the rat, although there is as yet no evidence for the existence of rat lambda 1-like proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1151-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Expression of two mRNAs for immunoglobulin lambda chains in the rat.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't