Linked Life Data

2447084

Source:http://linkedlifedata.com/resource/pubmed/id/2447084

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-2-25
pubmed:databankReference
pubmed:abstractText
Human hepatic lipase is an important enzyme in high density lipoprotein (HDL) metabolism, being implicated in the conversion of HDL2 to HDL3. Three human hepatic lipase cDNA clones were identified in two lambda gt11 libraries from human liver. The cDNA-derived amino acid sequence predicts a protein of 476 amino acid residues, preceded by a 23-residue signal peptide. Four potential N-glycosylation sites are identified, two of which are conserved in rat hepatic lipase. On alignment with human, mouse, and bovine lipoprotein lipase, the same two sites were also conserved in lipoprotein lipase in all three species. Stringent conservation of the cysteine residues was also evident. Comparative analysis of amino acid sequences shows that hepatic lipase evolves at a rapid rate, 2.07 x 10(-9) substitutions/site/year, about four times that in lipoprotein lipase and half that in pancreatic lipase. Further, hepatic lipase and pancreatic lipase appear to be evolutionarily closer to each other than either of them is to lipoprotein lipase. Southern blot analysis revealed high frequency restriction fragment length polymorphisms of the hepatic lipase gene for the enzymes HindIII and MspI. these polymorphisms will be useful for haplotype and linkage analysis of the hepatic lipase gene. Using cloned human hepatic lipase cDNA as a hybridization probe, we performed Southern blot analysis of a panel of 13 human-rodent somatic cell hybrids. Concordance analysis of the various hybrid clones indicates that the hepatic lipase gene is located on the long arm of human chromosome 15. Analysis of hybrids containing different translocations of chromosome 15 localized the gene to the region 15q15----q22.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1107-10
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2447084-Amino Acids, pubmed-meshheading:2447084-Animals, pubmed-meshheading:2447084-Base Sequence, pubmed-meshheading:2447084-Cattle, pubmed-meshheading:2447084-Chromosome Mapping, pubmed-meshheading:2447084-DNA, pubmed-meshheading:2447084-DNA Restriction Enzymes, pubmed-meshheading:2447084-Deoxyribonuclease HindIII, pubmed-meshheading:2447084-Deoxyribonuclease HpaII, pubmed-meshheading:2447084-Dogs, pubmed-meshheading:2447084-Humans, pubmed-meshheading:2447084-Lipase, pubmed-meshheading:2447084-Lipolysis, pubmed-meshheading:2447084-Lipoprotein Lipase, pubmed-meshheading:2447084-Liver, pubmed-meshheading:2447084-Mice, pubmed-meshheading:2447084-Molecular Sequence Data, pubmed-meshheading:2447084-Pancreas, pubmed-meshheading:2447084-Polymorphism, Genetic, pubmed-meshheading:2447084-Polymorphism, Restriction Fragment Length, pubmed-meshheading:2447084-Rats, pubmed-meshheading:2447084-Species Specificity, pubmed-meshheading:2447084-Swine
pubmed:year
1988
pubmed:articleTitle
Human hepatic lipase. Cloned cDNA sequence, restriction fragment length polymorphisms, chromosomal localization, and evolutionary relationships with lipoprotein lipase and pancreatic lipase.
pubmed:affiliation
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't