Linked Life Data

2446512

Source:http://linkedlifedata.com/resource/pubmed/id/2446512

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 2
pubmed:dateCreated
1988-1-12
pubmed:abstractText
The hypothesis that extracellular [Ca2+] ([Ca2+]e) influences the transmission of vasoregulatory information from the heart to coronary smooth muscle was examined. The isolated, retrogradely perfused rat heart was used to evaluate vascular reactivity to transient infusion of either 0.88 mM Amytal (amobarbital), hypoxia (95% N2-5% CO2), or 12 microM adenosine in the presence and absence of 2.5 microM verapamil. Amytal alone enhanced coronary flow by 78% and lactate efflux by 10-fold, whereas it decreased O2 consumption by 40% and the ratio of the free cytosolic concentrations of ATP-to-ADP times the intracellular concentration of inorganic phosphate ([ATP]f/[ADP]f[Pi]) by 10-fold. These responses were attenuated by preperfusion with verapamil, i.e., flow and lactate efflux increased by 4% and fourfold, respectively, and O2 consumption and [ATP]f/[ADP]f/[Pi] decreased by 8% and threefold, respectively, as compared with values attained with verapamil at steady state. Washout of the calcium channel antagonist by perfusion for 55 min with a verapamil-free medium resulted in a return of metabolic variables to control levels. Subsequent infusion of Amytal induced metabolic changes that were similar to those obtained before exposure to the calcium antagonist, but flow rose only by 26%. Vascular responses to hypoxia were similarly modified, whereas those to adenosine were unaffected. Hearts perfused with media containing either low [Ca2+]e or 10 microM diltiazem and exposed to Amytal produced flow patterns analogous to those perfused with verapamil. These data, in part, distinguish changes in vascular resistance that occur in response to cardiac metabolism from those which result from agents with direct action on smooth muscle, in particular, adenosine.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
253
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
H1098-105
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Relationship of myocardial metabolism and coronary flow: dependence on extracellular calcium.
pubmed:affiliation
Department of Biochemistry, University of Pennsylvania School of Medicine, Philadelphia 19104.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.