Linked Life Data

2445823

Source:http://linkedlifedata.com/resource/pubmed/id/2445823

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1988-1-4
pubmed:abstractText
A regulatory protein purified from rabbit erythrocyte stroma had decay-accelerating activity to C5 convertases on hemolytic intermediate cells (EAC1-3b, P). Its molecular weight was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under non-reducing conditions to be 66,000, slightly smaller than that of human decay-accelerating factor (DAF). The amino acid composition of the protein resembled that of human DAF. The protein was incorporated into sheep erythrocytes. The incorporation and the decay-accelerating activity were inhibited by low density lipoprotein. It did not bind to rabbit C3-Sepharose in a column under conditions in which human CR1 bound, but its interaction with rabbit C3 was evident from the retardation of its elution from the column. These results indicate that the protein purified from rabbit erythrocyte stroma was DAF.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Isolation of decay-accelerating factor (DAF) from rabbit erythrocyte membranes.
pubmed:affiliation
Department of Biochemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't