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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1987-11-25
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pubmed:databankReference |
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pubmed:abstractText |
Membrane glycoprotein (GP) IIb-IIIa is a component of a platelet adhesive protein receptor. A region of the heavy chain of GPIIb, defined by the monoclonal antibody PMI-1, is involved in adhesion receptor function. We have localized and chemically synthesized this region of GPIIb. A cDNA clone that directs the synthesis of a fusion protein reactive with the PMI-1 antibody was isolated from a phage lambda gt11 expression library constructed with mRNA from an erythroleukemia (HEL) cell line. The deduced amino acid sequence of this clone indicates that it spans the light-heavy chain junction of GPIIb and contains a portion of the carboxyl terminus of the heavy chain and the amino terminus of the light chain. The PMI-1 epitope was found to be contained within a 9-kDa staphylococcal V8 protease fragment of GPIIb, and such a fragment was predicted within the putative heavy-chain sequence. A computerized antigen prediction program identified a single sequence with a high probability of containing a continuous epitope. A synthetic 17-residue peptide containing this sequence binds PMI-1 and inhibits PMI-1 binding to GPIIb-IIIa. The peptide-antibody complex has an approximate Kd of 1.2 microM, which compares to a Kd of 0.95 microM for PMI-1 binding to GPIIb. The region containing the PMI-1 epitope shows no similarity to corresponding regions of two other adhesion receptors, indicating that this portion of GPIIb may function in activities unique to the platelet receptor.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-2412224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-2418980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-2420006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-2426709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-2428841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-2430295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-2437107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-2581314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-2944883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3006053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3010776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3028646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3155652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3458261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3487386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3494014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3534886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3546305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-3805026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-447655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6088559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6165051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6177045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6196430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6213621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6238974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6270087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6281889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6302680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6310559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6325435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6356359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6449521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6826726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-6999296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-7082398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2444974-7093203
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7114-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2444974-Amino Acid Sequence,
pubmed-meshheading:2444974-Antibodies, Monoclonal,
pubmed-meshheading:2444974-Base Sequence,
pubmed-meshheading:2444974-Binding Sites,
pubmed-meshheading:2444974-DNA,
pubmed-meshheading:2444974-Epitopes,
pubmed-meshheading:2444974-Humans,
pubmed-meshheading:2444974-Molecular Sequence Data,
pubmed-meshheading:2444974-Peptide Fragments,
pubmed-meshheading:2444974-Platelet Adhesiveness,
pubmed-meshheading:2444974-Platelet Membrane Glycoproteins,
pubmed-meshheading:2444974-Recombinant Fusion Proteins
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pubmed:year |
1987
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pubmed:articleTitle |
Molecular cloning and chemical synthesis of a region of platelet glycoprotein IIb involved in adhesive function.
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pubmed:affiliation |
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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