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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1987-11-25
|
| pubmed:abstractText |
The distribution of transglutaminases, the Ca2+-dependent protein cross-linking enzymes, in the human pituitary gland was investigated by immunohistological methods using specific antibodies. Tissue-type transglutaminase was specifically localized in ACTH-producing cells, and the cells producing GH, PRL, TSH, FSH, and LH contained no appreciable amount of the enzyme. No detectable plasma-type transglutaminase (coagulation factor XIII) was found in pituitary tissue. In a previous study we demonstrated that ACTH-producing cells contain very little Ca2+-dependent proteinases (calpain), but a remarkable amount of their inhibitor, calpastatin. Pituitary gland cells producing hormones other than ACTH contained calpains, but no detectable calpastatin. These results collectively suggest that intracellular substrate proteins in ACTH-producing cells are protected from Ca2+-dependent degradation and are substrates for Ca2+-dependent cross-linking catalyzed by the tissue-type transglutaminase. In other pituitary gland cells, conversely, the intracellular substrate proteins are more likely to undergo Ca2+-dependent degradation than cross-linking.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0021-972X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
65
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
885-90
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2444616-Adolescent,
pubmed-meshheading:2444616-Adrenocorticotropic Hormone,
pubmed-meshheading:2444616-Adult,
pubmed-meshheading:2444616-Aged,
pubmed-meshheading:2444616-Aged, 80 and over,
pubmed-meshheading:2444616-Antibodies,
pubmed-meshheading:2444616-Antibody Specificity,
pubmed-meshheading:2444616-Antigens,
pubmed-meshheading:2444616-Child,
pubmed-meshheading:2444616-Child, Preschool,
pubmed-meshheading:2444616-Female,
pubmed-meshheading:2444616-Humans,
pubmed-meshheading:2444616-Immunohistochemistry,
pubmed-meshheading:2444616-Infant,
pubmed-meshheading:2444616-Male,
pubmed-meshheading:2444616-Middle Aged,
pubmed-meshheading:2444616-Pituitary Gland,
pubmed-meshheading:2444616-Staining and Labeling,
pubmed-meshheading:2444616-Transglutaminases
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Specific localization of tissue-type transglutaminase in adrenocorticotropin-producing cells of the human pituitary gland as demonstrated by immunohistochemistry.
|
| pubmed:affiliation |
Department of Clinical Science, Kyoto University Faculty of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|