Linked Life Data

2443710

Source:http://linkedlifedata.com/resource/pubmed/id/2443710

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-11-12
pubmed:databankReference
pubmed:abstractText
Using purified RNA from HeLa cells, we have synthesized and cloned a cDNA encoding an almost entire 7 S K RNA. This cDNA probe was used to isolate 7 S K RNA gene sequences from a human genomic library by high-stringency colony hybridization. In order to differentiate between functional genes and related sequences, we have used a rapid in-vitro transcription assay of purified phage DNA. With this additional screening criterion applied to selected clones, we have obtained one recombinant phage that contained a complete 7 S K RNA gene and, immediately adjacent to its 3' end, a truncated pseudogene. The nucleotide sequence of both genes including the flanking regions has been determined. The functional integrity of the isolated 7 S K RNA gene was verified by in-vitro transcription studies with cell-free extracts and by fingerprinting of the specific transcripts with ribonuclease T1. Under optimal ionic conditions, the transcription efficiency in vitro of this 7 S K RNA gene was found to be comparable to that of a human 7 S K RNA in vitro depends on 5'-flanking sequences. The region up to position -67 was determined to be essential for efficient transcription in vitro of 7 S K RNA. While apparently a variety of 7 S K related sequences is distributed within the human genome, hybridization of 5'-flanking sequences to genomic DNA revealed that possibly not more than one copy of this gene is present per haploid genome.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-2836
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
195
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Structural and functional analysis of a human 7 S K RNA gene.
pubmed:affiliation
Department of Biochemistry, Ruhr-University, Bochum, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't