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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1987-11-17
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pubmed:abstractText |
1. The single 'giga-seal' patch-electrode technique (Hamill, Marty, Neher, Sakmann & Sigworth, 1981) was used to record whole-cell currents in the GH3 rat anterior pituitary cell line. 2. GH3 cells have a rapidly inactivating, voltage-dependent K+ current that is selectively inhibited by 4-aminopyridine (4-AP) but not by tetraethylammonium chloride (TEA). 3. The majority of the Ca2+-activated K+ current in these cells is blocked by TEA with an inhibitory concentration that is half-maximal at 1 mM. An additional Ca2+-activated K+ current is also present that is relatively resistant to TEA and is blocked by the polypeptide apamin. The apamin-sensitive component represents less than 18% of the total Ca2+-activated K+ current at 0 mV. 4. The time course of the slowly declining components of the Ca2+-activated K+ tail currents measured at the -50 mV holding potential was usually biexponential with time constants of 0.21 +/- 0.02 and 1.75 +/- 0.23 s (mean +/- S.E. of mean, n = 14). Both of the two slowly decaying components contribute to the TEA- and apamin-sensitive currents. 5. It is concluded that GH3 cells have at least two pharmacologically distinct Ca2+-activated K+ currents and a 4-AP-sensitive voltage-dependent K+ current.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-1177091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-19431572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-2409514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-2419479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-2443674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-2579386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-2582121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-480244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-4951281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-533944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-5575340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6086805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6086894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6097678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6122211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6251744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6273550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6278324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6280066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6285269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6291655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6296366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6296367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6300404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6302197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6306751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6308233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6308236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6322759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6323003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6325585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6325587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6411494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6482969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-6769299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-7109830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-7284554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-813152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443673-850203
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
385
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
591-609
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:2443673-4-Aminopyridine,
pubmed-meshheading:2443673-Action Potentials,
pubmed-meshheading:2443673-Aminopyridines,
pubmed-meshheading:2443673-Animals,
pubmed-meshheading:2443673-Apamin,
pubmed-meshheading:2443673-Calcium,
pubmed-meshheading:2443673-Cell Line,
pubmed-meshheading:2443673-Depression, Chemical,
pubmed-meshheading:2443673-Edetic Acid,
pubmed-meshheading:2443673-Ion Channels,
pubmed-meshheading:2443673-Pituitary Gland, Anterior,
pubmed-meshheading:2443673-Potassium,
pubmed-meshheading:2443673-Rats,
pubmed-meshheading:2443673-Tetraethylammonium,
pubmed-meshheading:2443673-Tetraethylammonium Compounds
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pubmed:year |
1987
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pubmed:articleTitle |
Two distinct calcium-activated potassium currents in a rat anterior pituitary cell line.
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pubmed:affiliation |
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77550.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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