Linked Life Data

2443484

Source:http://linkedlifedata.com/resource/pubmed/id/2443484

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1987-11-5
pubmed:abstractText
We characterized the in vivo transcription of the Cellulomonas fimi cenB gene, which encodes an extracellular endo-beta-1,4-glucanase (EC 3.2.1.4). By Northern blot (RNA blot) analysis, cenB mRNA was detected in C. fimi RNA preparations from glycerol-, glucose-, and carboxymethyl cellulose (CMC)-grown cells. The relative abundance of the specific mRNAs in these preparations appeared to depend on the carbon source provided, with the preparations from CMC-grown cells having the greatest amount of cenB transcripts, followed by glycerol- and glucose-grown cells. Therefore, the transcription of this gene could be regulated by the carbon source provided to C. fimi. High-resolution nuclease S1 protection studies were used to map cenB mRNA 5' termini with a unique 5'-labeled DNA probe and C. fimi RNA isolated in vivo. With this procedure, three 5' termini were found in abundance upstream of the translational initiation ATG codon in RNA preparations from C. fimi grown on CMC, while less-abundant 5' termini were found 52 bases closer to the ATG codon in RNA prepared from C. fimi grown on any one of the three substrates. These results are indicative of a tandem promoter arrangement, with the ATG-proximal promoter directing constitutive low-level cenB transcription and the more distal promoter directing higher levels of transcription under the inducing effects of the cellulosic substrate. The corresponding transcripts were not detected in S1 mapping experiments with RNA isolated in vivo from Escherichia coli clones harboring recombinant plasmids carrying C. fimi genomic inserts. Comparative analysis of the 5' -flanking DNA sequences of the cenB gene and the cenA and cex genes of C. fimi (N. M. Greenberg, R. A. J. Warren, D. G. Kilburn, and R. C. Miller, Jr., J. Bacteriol. 169:646-653, 1987) revealed a region of 50 bases in which these sequences displayed at least 64% homology.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-13718526, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-3023193, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-3031664, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-3096818, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-344137, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-3804971, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-6246368, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-6295879, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-6310323, http://linkedlifedata.com/resource/pubmed/commentcorrection/2443484-922889
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
169
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4674-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Regulation and initiation of cenB transcripts of Cellulomonas fimi.
pubmed:affiliation
Department of Microbiology, University of British Columbia, Vancouver, Canada.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't