Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1987-10-5
|
| pubmed:abstractText |
Contamination of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) samples with skin keratin has produced a recurrent problem of artefactual bands when highly sensitive detection methods, such as silver staining, have been used. Such an artefact also can be a problem in immunoblots because some sera appear to contain antibodies that react with a human skin keratin. A simple method for the removal of these antibodies with keratin immobilized on an affinity chromatography substrate is described.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0022-1759
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
102
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
143-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1987
|
| pubmed:articleTitle |
Elimination of the detection of an artefactual 65 kDa keratin band from immunoblots.
|
| pubmed:publicationType |
Journal Article
|